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Journal of Clinical Microbiology, January 2006, p. 254-256, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.254-256.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Safe Mycobacterium tuberculosis DNA Extraction Method That Does Not Compromise Integrity

Robin Warren,* Marianna de Kock, Erica Engelke, Roseline Myburgh, Nico Gey van Pittius, Thomas Victor, and Paul van Helden

DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa

Received 13 September 2005/ Returned for modification 11 October 2005/ Accepted 18 October 2005

Concern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosis without compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.

* Corresponding author. Mailing address: DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry, Faculty of Health Sciences, Stellenbosch University, P.O. Box 19063, Tygerberg 7505, South Africa. Phone: 021-9389482. Fax: 021-9389476. E-mail: rw1{at}sun.ac.za.

Journal of Clinical Microbiology, January 2006, p. 254-256, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.254-256.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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