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Journal of Clinical Microbiology, January 2006, p. 257-259, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.257-259.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Utility of Extended Blood Culture Incubation for Isolation of Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella Organisms: a Retrospective Multicenter Evaluation

Cathy A. Petti,1* Hasan S. Bhally,2,3,{dagger} Melvin P. Weinstein,3 Kim Joho,3 Teresa Wakefield,2 L. Barth Reller,4 and Karen C. Carroll2

Departments of Medicine and Pathology, University of Utah School of Medicine, Salt Lake City, Utah,1 Johns Hopkins Medical Institutions, Baltimore, Maryland,2 Robert Wood Johnson Medical School, New Brunswick, New Jersey,3 Departments of Medicine and Pathology and Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina4

Received 1 September 2005/ Accepted 14 October 2005

The incidence of and average time to detection for Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella (HACEK) bacteria in blood cultures with standard incubation and the utility of extended incubation of blood culture bottles were reviewed at four tertiary care microbiology laboratories. HACEK organisms were isolated from 35 (<0.005%) of 59,203 positive blood cultures. None of 407 blood cultures with extended incubation grew HACEK or other bacteria. Bacteremia from HACEK bacteria is rare, and extended incubation of blood cultures to recover HACEK bacteria is unnecessary.

* Corresponding author. Mailing address: ARUP Laboratories, Inc., 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787. Fax: (801) 584-5207. E-mail: cathy.petti{at}aruplab.com.

{dagger} Current address: Internal Medicine and Infectious Diseases, Waitemata District Health Board, Department of Medicine, Level 3, North Shore Hospital, Private Bag 93-503, Shakespeare Road, Takapuna, Auckland 9, New Zealand.

Journal of Clinical Microbiology, January 2006, p. 257-259, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.257-259.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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