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Journal of Clinical Microbiology, January 2006, p. 260-265, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.260-265.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multiple-Locus Variable-Number Tandem Repeat Analysis for Typing of Staphylococcus epidermidis

Department of Clinical Microbiology, Infectious Diseases,¹ Department of Clinical Microbiology, Bacteriology, Umeå University, SE-901 85 Umeå,³ Department of NBC-Analysis, Swedish Defence Research Agency, SE-901 82 Umeå, Sweden²

Received 22 June 2005/ Returned for modification 29 July 2005/ Accepted 3 November 2005

We applied a high-resolution PCR-based typing method, multiple-locus variable-number tandem repeat analysis (MLVA), for discrimination of 30 multidrug-resistant clinical isolates of Staphylococcus epidermidis. The results of MLVA were congruent with results obtained by pulsed-field gel electrophoresis (PFGE). MLVA generated discrete character data, and its discriminatory capacity was comparable to that of PFGE.

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