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Journal of Clinical Microbiology, January 2006, p. 260-265, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.260-265.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multiple-Locus Variable-Number Tandem Repeat Analysis for Typing of Staphylococcus epidermidis

Anders Johansson,1,2* Satu Koskiniemi,3 Per Gottfridsson,3 Johan Wiström,1 and Tor Monsen3

Department of Clinical Microbiology, Infectious Diseases,1 Department of Clinical Microbiology, Bacteriology, Umeå University, SE-901 85 Umeå,3 Department of NBC-Analysis, Swedish Defence Research Agency, SE-901 82 Umeå, Sweden2

Received 22 June 2005/ Returned for modification 29 July 2005/ Accepted 3 November 2005

We applied a high-resolution PCR-based typing method, multiple-locus variable-number tandem repeat analysis (MLVA), for discrimination of 30 multidrug-resistant clinical isolates of Staphylococcus epidermidis. The results of MLVA were congruent with results obtained by pulsed-field gel electrophoresis (PFGE). MLVA generated discrete character data, and its discriminatory capacity was comparable to that of PFGE.

* Corresponding author. Mailing address: NBC-Analysis, Swedish Defence Research Agency, SE-901 82 Umeå, Sweden. Phone: 46 90 10 66 16. Fax: 46 90 13 30 06. E-mail: anders.johansson{at}infdis.umu.se.

Journal of Clinical Microbiology, January 2006, p. 260-265, Vol. 44, No. 1
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.1.260-265.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, M., Wang, X., Gao, Q., Lu, Y. (2009). Molecular characterization of Staphylococcus epidermidis strains isolated from a teaching hospital in Shanghai, China. J Med Microbiol 58: 456-461 [Abstract] [Full Text]  
  • Monk, A. B., Archer, G. L. (2007). Use of Outer Surface Protein Repeat Regions for Improved Genotyping of Staphylococcus epidermidis. J. Clin. Microbiol. 45: 730-735 [Abstract] [Full Text]  


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