Previous Article | Next Article ![]()
Journal of Clinical Microbiology, January 2006, p. 77-84, Vol. 44, No. 1
0095-1137/06/$08.00+0 doi:10.1128/JCM.44.1.77-84.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
and
P. P. Bosshard*
Institut für Medizinische Mikrobiologie, Universität Zürich, 8006 Zürich, Switzerland
Received 28 June 2005/ Returned for modification 15 August 2005/ Accepted 12 October 2005
In this study, we established an in-house database of yeast internal transcribed spacer (ITS) sequences. This database includes medically important as well as colonizing yeasts that frequently occur in the diagnostic laboratory. In a prospective study, we compared molecular identification with phenotypic identification by using the ID32C system (bioMérieux) for yeast strains that could not be identified by a combination of CHROMagar Candida and morphology on rice agar. In total, 113 yeast strains were included in the study. By sequence analysis, 98% of all strains were identified correctly to the species level. With the ID32C, 87% of all strains were identified correctly to the species or genus level, 7% of the isolates could not be identified, and 6% of the isolates were misidentified, most of them as Candida rugosa or Candida utilis. For a diagnostic algorithm, we suggest a three-step procedure which integrates morphological criteria, biochemical investigation, and sequence analysis of the ITS region.
Present address: Bio-Analytica AG, 6000 Luzern 6, Switzerland.
This article has been cited by other articles:
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»