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Journal of Clinical Microbiology, October 2006, p. 3493-3498, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00724-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mycobacterium barrassiae sp. nov., a Mycobacterium moriokaense Group Species Associated with Chronic Pneumonia

Toïdi Adékambi, Didier Raoult, and Michel Drancourt*

Unité des Rickettsies, CNRS UMR 6020 IFR 48, Faculté de Médecine, Université de la Méditerranée, and Assistance Publique-Hôpitaux de Marseille Timone Fédération de Microbiologie Clinique Marseille, France

Received 5 April 2006/ Returned for modification 16 June 2006/ Accepted 25 July 2006

Three identical isolates of new rapidly growing mycobacteria (RGM) were recovered from the bronchial aspirate and sputum from a 49-year-old woman presenting with lung lesions. The case met the American Thoracic Society criteria for the diagnosis of nontuberculous mycobacterial infection. The three isolates grew in 3 days at 24 to 42°C. The 16S rRNA gene sequence analysis indicated that the sequences of the isolates were identical and shared 99.7% and 98.1% similarities with those of Mycobacterium moriokaense and Mycobacterium gadium, respectively. Partial 723-bp rpoB sequence analyses indicated that the sequences of the isolates shared 95.8% and 92.3% similarities with those of M. moriokaense and M. gadium, respectively. Polyphasic identification (including biochemical tests; antimicrobial susceptibility profiling; and hsp65, recA, and sodA gene sequence analyses, as well as G+C content determination and cell wall fatty acid composition analysis) supported the evidence that these isolates were representative of a new species. Phylogenetic analyses confirmed the close relationships of the isolates with M. moriokaense and the defined M. moriokaense group. These isolates were susceptible to the antimicrobials currently recommended for the treatment of RGM infections. These isolates differed from M. moriokaense by their susceptibility to vancomycin. We propose the name Mycobacterium barrassiae sp. nov. for this new species. The type strain is N7T (CIP 108545T and CCUG 50398T).

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27, Université de la Méditerranée, Boulevard Jean Moulin, 13385 Marseille Cedex 05, France. Phone: (33).04.91.32.43.75. Fax: (33).04.91.38.77.72. E-mail: Michel.Drancourt{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, October 2006, p. 3493-3498, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00724-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Simmon, K. E., Low, Y. Y., Brown-Elliott, B. A., Wallace, R. J. Jr, Petti, C. A. (2009). Phylogenetic analysis of Mycobacterium aurum and Mycobacterium neoaurum with redescription of M. aurum culture collection strains. Int. J. Syst. Evol. Microbiol. 59: 1371-1375 [Abstract] [Full Text]  
  • Adekambi, T., Shinnick, T. M., Raoult, D., Drancourt, M. (2008). Complete rpoB gene sequencing as a suitable supplement to DNA-DNA hybridization for bacterial species and genus delineation. Int. J. Syst. Evol. Microbiol. 58: 1807-1814 [Abstract] [Full Text]  


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