JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JCM.00701-06v1
44/10/3608    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, S.
Right arrow Articles by Boyle, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, S.
Right arrow Articles by Boyle, D. S.
Journal of Clinical Microbiology, October 2006, p. 3608-3615, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00701-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multiplex PCR-Based Method for Identification of Common Clinical Serotypes of Salmonella enterica subsp. enterica{triangledown}

Seonghan Kim,1 Jonathan G. Frye,2 Jinxin Hu,1 Paula J. Fedorka-Cray,2 Romesh Gautom,1 and David S. Boyle1*

Washington State Department of Health, Public Health Laboratories, 1610 NE 150th St., Shoreline, Washington 98155-7224,1 Bacterial Epidemiology and Antimicrobial Resistance Research Unit, U.S. Department of Agriculture, Agricultural Research Service, 950 College Station Road, Athens, Georgia 306052

Received 3 April 2006/ Returned for modification 16 May 2006/ Accepted 8 August 2006

A multiplex PCR method has been developed to differentiate between the most common clinical serotypes of Salmonella enterica subsp. enterica encountered in Washington State and the United States in general. Six genetic loci from S. enterica serovar Typhimurium and four from S. enterica serovar Typhi were used to create an assay consisting of two five-plex PCRs. The assays gave reproducible results with 30 different serotypes that represent the most common clinical isolates of S. enterica subsp. enterica. Of these, 22 serotypes gave unique amplification patterns compared with each other and the other 8 serotypes were grouped into four pairs. These were further resolved by two additional PCRs. We compared the data from PCR serotyping with conventional serotyping and found that PCR serotyping was nearly as discriminatory as conventional serotyping was. The results from a blind test screening 111 clinical isolates revealed that 97% were correctly identified using the multiplex PCR assay. The assay can be easily performed on multiple samples with final results in less than 5 h and, in conjunction with pulsed-field gel electrophoresis, forms a very robust test method for the molecular subtyping of Salmonella enterica subsp. enterica.

* Corresponding author. Mailing address: Washington State Department. of Health, Public Health Laboratories, 1610 NE 150th St., Shoreline, WA 98155-7224. Phone: (206) 418-5462. Fax: (206) 418-5545. E-mail: david.boyle{at}doh.wa.gov.

{triangledown} Published ahead of print on 30 August 2006.

Journal of Clinical Microbiology, October 2006, p. 3608-3615, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.00701-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2006 by the American Society for Microbiology. All rights reserved.