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Journal of Clinical Microbiology, October 2006, p. 3674-3679, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01087-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Development of an Enzyme Immunoassay for Detection of Sapovirus-Specific Antibodies and Its Application in a Study of Seroprevalence in Children

Tibor Farkas,1,3* Xiaoyun Deng,1 Guillermo Ruiz-Palacios,4 Ardythe Morrow,2,3 and Xi Jiang1,3

Division of Infectious Diseases,1 Center for Epidemiology and Biostatistics, Cincinnati Children's Hospital Medical Center,2 Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio,3 Department of Infectious Diseases, Institute of Medical Services and Nutrition, Mexico City, Mexico4

Received 25 May 2005/ Returned for modification 25 July 2006/ Accepted 4 August 2006

Sapoviruses (SVs) are an important cause of acute pediatric gastroenteritis. Due to the lack of appropriate diagnostic methods, the epidemiology of SV-associated illness remains poorly understood. Baculovirus and Escherichia coli expression systems were evaluated for the development of antibody detection enzyme immunoassays (EIA). Age-related antibody prevalence in children was studied using the new EIA. Because of the low yield of the baculovirus system, the E. coli-expressed SV capsid proteins were used to develop the EIA. The antigenic specificities of the E. coli-expressed SV capsid proteins were demonstrated using hyperimmune antisera raised in animals and sera collected from patients. A high prevalence (>90%) of antibodies to both SV (strain Mex340) and norovirus (strain VA387) was observed in children involved in a birth cohort at 20 to 24 months of age; however, at 1 to 3 months of age, <25% of the children possessed anti-SV antibodies versus >90% with anti-NV antibodies. The E. coli-derived SV proteins are an excellent source of antigens for the EIA. SV infection is common in the first 2 years of life. The low prevalence of maternal antibodies detected in Mexican children against SVs in this study is unique and needs to be addressed in future studies.

* Corresponding author. Mailing address: Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229. Phone: (513) 636-0131. Fax: (513) 636-7655. E-mail: Tibor.Farkas{at}cchmc.org.

Journal of Clinical Microbiology, October 2006, p. 3674-3679, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01087-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, W., Shen, Q., Hua, X., Cui, L., Liu, J., Yang, S. (2008). The First Chinese Porcine Sapovirus Strain That Contributed to an Outbreak of Gastroenteritis in Piglets. J. Virol. 82: 8239-8240 [Full Text]  


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