This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mueller, M. Articles by Hartung, T. Search for Related Content PubMed PubMed Citation Articles by Mueller, M. Articles by Hartung, T.

Identification of Borrelia burgdorferi Ribosomal Protein L25 by the Phage Surface Display Method and Evaluation of the Protein's Value for Serodiagnosis

Markus Mueller,^1, Sebastian Bunk,^1, Isabel Diterich,¹ Michael Weichel,² Carolin Rauter,¹ Dieter Hassler,³ Corinna Hermann,¹ Reto Crameri,² and Thomas Hartung^1,4*

University of Konstanz, Biochemical Pharmacology, Konstanz, Germany,¹ Swiss Institute of Allergy and Asthma Research, Daros, Switzerland,² Untere Hofstaat 3, Kraichtal, Germany,³ ECVAM, EU Joint Research Centre, IHCP, Ispra, Italy⁴

Received 20 February 2006/ Returned for modification 19 April 2006/ Accepted 14 July 2006

The phage surface display technique was used to identify Borrelia burgdorferi antigens. By affinity selection with immunoglobulin G from pooled sera of six Lyme borreliosis (LB) patients, the ribosomal protein L25 was identified. The diagnostic value of L25 was investigated by an enzyme-linked immunosorbent assay, using sera from 80 LB patients and 75 controls, and the use of the protein resulted in a specificity of 99% and a 23% sensitivity, which qualify L25 as a useful antigen when combined with others.

M.M. and S.B. contributed equally to this study.