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Journal of Clinical Microbiology, October 2006, p. 3822-3825, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01232-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of a Multiplex PCR-Based Reverse Line Blot-Hybridization Assay for Identification of Serotype and Surface Protein Antigens of Streptococcus agalactiae

Xianyu Zeng,1,2 Fanrong Kong,1 Julie Morgan,3 and Gwendolyn L. Gilbert1*

Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales, Australia,1 Department of Dermatology, Wuhan First Hospital, Wuhan, Hubei Province, People's Republic of China,2 Streptococcus Reference Laboratory, Institute of Environmental Science and Research Ltd., Porirua, New Zealand3

Received 15 June 2006/ Accepted 22 June 2006

A 33-primer multiplex PCR-based reverse line blot (mPCR/RLB) assay was developed to identify Streptococcus agalactiae serotypes and surface protein antigen genes simultaneously. It was evaluated by using 551 clinical isolates. The mPCR/RLB assay was more sensitive than conventional serotyping, especially for protein antigen typing, but otherwise the results correlated well.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, Darcy Road, Westmead, New South Wales 2145, Australia. Phone: (612) 9845 6255. Fax: (612) 9893 8659. E-mail: lyng{at}icpmr.wsahs.nsw.gov.au.

Journal of Clinical Microbiology, October 2006, p. 3822-3825, Vol. 44, No. 10
0095-1137/06/$08.00+0     doi:10.1128/JCM.01232-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kong, F., Lambertsen, L. M., Slotved, H.-C., Ko, D., Wang, H., Gilbert, G. L. (2008). Use of Phenotypic and Molecular Serotype Identification Methods To Characterize Previously Nonserotypeable Group B Streptococci. J. Clin. Microbiol. 46: 2745-2750 [Abstract] [Full Text]  
  • Slotved, H.-C., Kong, F., Lambertsen, L., Sauer, S., Gilbert, G. L. (2007). Serotype IX, a Proposed New Streptococcus agalactiae Serotype. J. Clin. Microbiol. 45: 2929-2936 [Abstract] [Full Text]  


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