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Journal of Clinical Microbiology, November 2006, p. 3994-3999, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00952-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Methods for Detection of Trichomonas vaginalis in the Male Partners of Infected Women: Implications for Control of Trichomoniasis{triangledown}

Marcia M. Hobbs,1,2* Dana M. Lapple,1 Lisa F. Lawing,4 Jane R. Schwebke,4 Myron S. Cohen,1,2 Heidi Swygard,1 Julius Atashili,3 Peter A. Leone,1,6 William C. Miller,1,3 and Arlene C. Seña1,5

Departments of Medicine,1 Microbiology and Immunology,2 Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina,3 Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, Alabama,4 Durham County Health Department, Durham, North Carolina,5 Wake County Department of Health and Human Services, Raleigh, North Carolina6

Received 6 May 2006/ Returned for modification 22 June 2006/ Accepted 30 August 2006

Trichomonas vaginalis infection in men is an important cause of nongonococcal urethritis. Effective detection of the parasite in men using culture requires examination of multiple specimens. We compared culture and PCR-enzyme-linked immunosorbent assay in urethral swabs, urine, and semen for T. vaginalis detection in male sexual partners of women with trichomoniasis identified by wet mount and culture. Trichomonads were detected by at least one positive test in 205/280 men (73.2%) who submitted at least one specimen for culture and PCR. Whereas InPouch TV culture detected only 46/205 cases (22.5%), PCR detected 201/205 (98.0%). Urethral swab cultures from men with urethritis were more likely to be positive with shorter incubation than specimens from men without urethritis. T. vaginalis was detected more often in men with wet-mount-positive partners. Even with a sensitive PCR assay, reliable detection of T. vaginalis in male partners required multiple specimens. The majority of male sexual partners in this study were infected, emphasizing the importance of partner evaluation and treatment.

* Corresponding author. Mailing address: 103 Mason Farm Rd, 8309 MBRB, UNC CB# 7031, Chapel Hill, NC 27599. Phone: (919) 843-6893. Fax: (919) 843-1015. E-mail: mmhobbs{at}med.unc.edu.

{triangledown} Published ahead of print on 13 September 2006.

Journal of Clinical Microbiology, November 2006, p. 3994-3999, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00952-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Campbell, L., Woods, V., Lloyd, T., Elsayed, S., Church, D. L. (2008). Evaluation of the OSOM Trichomonas Rapid Test versus Wet Preparation Examination for Detection of Trichomonas vaginalis Vaginitis in Specimens from Women with a Low Prevalence of Infection. J. Clin. Microbiol. 46: 3467-3469 [Abstract] [Full Text]  


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