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Journal of Clinical Microbiology, November 2006, p. 4077-4084, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00017-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of a Multiple Restriction Fragment Length Polymorphism Method for Detecting Vaccine-Derived Polioviruses in Clinical Samples

Natalia I. Romanenkova,^1* Sophie Guillot,² Nadejda R. Rozaeva,¹ Radu Crainic,² Maina A. Bichurina,¹ and Francis Delpeyroux^2*

Subnational Poliomyelitis Laboratory, Saint-Petersburg Pasteur Institute, St. Petersburg, Russia,¹ Unit of Molecular Prevention and Therapy of Human Diseases, CNRS FRE 2849, Pasteur Institute, Paris, France²

Received 4 January 2006/ Returned for modification 1 March 2006/ Accepted 29 August 2006

Since the announcement of the WHO program for the global eradication of poliomyelitis and the establishment of epidemiological and virological surveillance, the emergence and circulation of pathogenic vaccine-derived polioviruses (VDPV) presenting >1% nucleotide divergence from the sequence of the original vaccine strain have been demonstrated in certain regions. We developed and used a multiple restriction fragment length polymorphism (RFLP) method to investigate the frequency of these VDPV in a population with a high level of oral poliovirus vaccine coverage in northwestern Russia. Modified RFLP profiles were found to be strongly correlated with the presence of mutations and recombination events in vaccine strains. We found that a substantial proportion of vaccine strains had high percentages of nucleotide substitutions (>0.5%), including a type 3 VDPV with 1.4% nucleotide divergence. These findings indicate that VDPV or pre-VDPV strains are not rare in certain populations with high levels of vaccine coverage. The multiple RFLP method appears to be a simple and rapid tool for monitoring such strains, which could jeopardize the benefits of the eradication program.

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* Corresponding author. Mailing address: Unité de Prévention et Thérapie Moléculaires des Maladies Humaines, Institut Pasteur, 75 724 Paris Cedex 15, France. Phone: 33 1 40 61 33 22. Fax: 33 1 45 68 87 80. E-mail for Francis Delpeyroux: delpeyro{at}pasteur.fr. E-mail for Natalia I. Romanenkova: poliospb{at}pasteur.org.ru.

Published ahead of print on 6 September 2006.

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Journal of Clinical Microbiology, November 2006, p. 4077-4084, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00017-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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