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Detection of Severe Acute Respiratory Syndrome Coronavirus in Stool Specimens by Commercially Available Real-Time Reverse Transcriptase PCR Assays

Sunnybrook Health Sciences Centre, Toronto, Ontario,¹ St. Joseph's Healthcare, Hamilton, Ontario,² Hospital for Sick Children, Toronto, Ontario,³ the Toronto Medical Laboratories and Mount Sinai Hospital, Toronto, Ontario,⁴ St. Michael's Hospital,⁵ the Central Public Health Laboratory, Toronto, Ontario,⁶ the University of Toronto, Toronto, Ontario,⁷ the Provincial Laboratory for Public Health and the University of Calgary, Calgary, Alberta, Canada⁸

Received 12 June 2006/ Accepted 21 August 2006

Three commercially available real-time reverse transcriptase PCR assays (the Artus RealArt HPA coronavirus LightCycler, the Artus RealArt HPA coronavirus Rotor-Gene, and the EraGen severe acute respiratory syndrome coronavirus POL assay) and three RNA extraction methodologies were evaluated for the detection of severe acute respiratory syndrome coronavirus RNA from 91 stool specimens. The assays' sensitivities were highest (58% to 75%) for specimens obtained 8 to 21 days after symptom onset. The assays were less sensitive when specimens were obtained less than 8 days or more than 21 days after the onset of symptoms. All assays were 100% specific.

Published ahead of print on 30 August 2006.

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