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Journal of Clinical Microbiology, November 2006, p. 4197-4199, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01447-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison between the Gen-Probe Transcription-Mediated Amplification Trichomonas vaginalis Research Assay and Real-Time PCR for Trichomonas vaginalis Detection Using a Roche LightCycler Instrument with Female Self-Obtained Vaginal Swab Samples and Male Urine Samples{triangledown}

Andrew Hardick, Justin Hardick, Billie Jo Wood, and Charlotte Gaydos*

Division of Infectious Diseases, Johns Hopkins University, School of Medicine, Baltimore, Maryland

Received 12 July 2006/ Accepted 21 August 2006

This study compared two assays for Trichomonas vaginalis detection, Gen-Probe's transcription-mediated amplification (TMA) assay for Trichomonas vaginalis and BTUB FRET PCR, using self-obtained clinical samples from 611 patients. Infection status was defined as two positive results by two different tests. The initial TMA assay sensitivity was 96.7%; specificity was 97.5%. The TMA assay was comparable to BTUB FRET PCR.

* Corresponding author. Mailing address: Division of Infectious Diseases, School of Medicine, Johns Hopkins University, 1159 Ross Building, 720 Rutland Ave., Baltimore, MD 21015. Phone: (410) 614-0932. Fax: (410) 614-9775. E-mail: cgaydos{at}jhmi.edu.

{triangledown} Published ahead of print on 30 August 2006.

Journal of Clinical Microbiology, November 2006, p. 4197-4199, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01447-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pattullo, L., Griffeth, S., Ding, L., Mortensen, J., Reed, J., Kahn, J., Huppert, J. (2009). Stepwise Diagnosis of Trichomonas vaginalis Infection in Adolescent Women. J. Clin. Microbiol. 47: 59-63 [Abstract] [Full Text]  
  • Campbell, L., Woods, V., Lloyd, T., Elsayed, S., Church, D. L. (2008). Evaluation of the OSOM Trichomonas Rapid Test versus Wet Preparation Examination for Detection of Trichomonas vaginalis Vaginitis in Specimens from Women with a Low Prevalence of Infection. J. Clin. Microbiol. 46: 3467-3469 [Abstract] [Full Text]  
  • Munson, E., Napierala, M., Olson, R., Endes, T., Block, T., Hryciuk, J. E., Schell, R. F. (2008). Impact of Trichomonas vaginalis Transcription-Mediated Amplification-Based Analyte-Specific-Reagent Testing in a Metropolitan Setting of High Sexually Transmitted Disease Prevalence. J. Clin. Microbiol. 46: 3368-3374 [Abstract] [Full Text]  
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