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Journal of Clinical Microbiology, November 2006, p. 4200-4203, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01218-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Presence of Pathogenicity Island Genes in Enterococcus faecalis Isolates from Pigs in Denmark{triangledown}

Nathan Shankar,1 Arto S. Baghdayan,1 Rob Willems,2 Anette M. Hammerum,3 and Lars B. Jensen4*

University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma,1 University Medical Center Utrecht, Utrecht, The Netherlands,2 Statens Serum Institut, Copenhagen, Denmark,3 Danish Institute for Food and Veterinary Research, Copenhagen, Denmark4

Received 14 June 2006/ Returned for modification 5 August 2006/ Accepted 26 August 2006

Enterococcus faecalis isolates of porcine origin were screened for the presence of a previously identified pathogenicity island (PAI). By using the esp gene as a genetic marker for the presence of this PAI, 9 esp-positive and 10 esp-negative isolates of porcine origin were investigated by use of a designed oligonucleotide array. The results indicated the clustering of esp-positive strains by multilocus sequence typing (MLST), but surprisingly, all strains investigated contained parts of the PAI. None of the strains of animal origin investigated belonged to previously identified MLST complex 2, where most isolates from patients cluster. Five of the nine esp-positive E. faecalis isolates of animal origin belonged to the same PAI complex as human isolate MMH594 but differed in their sequence types, which strongly indicates the horizontal transfer of the PAI between enterococci of porcine and human origin.

* Corresponding author. Mailing address: Antimicrobial Resistance Unit, Danish Institute for Food and Veterinary Research, 27 Bülowsvej, DK-1790 Copenhagen V, Denmark. Phone: 45 72 34 62 62. Fax: 45 72 34 60 01. E-mail: lje{at}dfvf.dk.

{triangledown} Published ahead of print on 6 September 2006.

Journal of Clinical Microbiology, November 2006, p. 4200-4203, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01218-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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