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Journal of Clinical Microbiology, November 2006, p. 4218-4221, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01216-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of Clinical Isolates of Mycobacterium bovis Recovered from Humans in Italy

Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, I-56127 Pisa,¹ Centro Regionale di Riferimento per i Micobatteri, Laboratorio di Microbiologia e Virologia, Ospedale Careggi, I-50134 Firenze, Italy²

Received 14 June 2006/ Returned for modification 29 August 2006/ Accepted 9 September 2006

In order to achieve a better knowledge of Mycobacterium bovis epidemiology in Italy, 42 clinical isolates from humans were genotyped. Predominant molecular patterns were found in one cluster of 15 isolates sharing spoligotype (ST482), variable-number tandem repeat (VNTR), and IS6110-based restriction fragment length polymorphism (one 1.9-kb band) profiles and in two clusters of 6 and 3 Mycobacterium bovis BCG isolates differing by one VNTR character. The remaining 18 isolates yielded unique profiles. Our results confirm the potential utility of spoligotyping and VNTR typing as a major typing system of M. bovis isolates.

Published ahead of print on 20 September 2006.