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Journal of Clinical Microbiology, November 2006, p. 4237-4241, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01512-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

MultiCode-RTx Real-Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples{triangledown}

Evguenia S. Svarovskaia,1* Michael J. Moser,2 Andrew S. Bae,1 James R. Prudent,2 Michael D. Miller,3 and Katyna Borroto-Esoda1

Gilead Sciences, Inc., Durham, North Carolina,1 EraGen Biosciences, Inc., Madison, Wisconsin,2 Gilead Sciences, Inc., Foster City, California3

Received 21 July 2006/ Returned for modification 24 August 2006/ Accepted 12 September 2006

We report a real-time PCR assay capable of detecting drug-resistant human immunodeficiency virus type 1 reverse transcriptase K65R mutant virus at a level of 0.5% in polymorphic patient plasma specimens. Fifty-three treatment-naïve and 20 treatment-experienced specimens were successfully genotyped with the new method. Results were in agreement with population sequencing and the labor-intensive single-genome sequencing method.

* Corresponding author. Mailing address: Gilead Sciences, Inc., 4 University Place, 4611 University Drive, Durham, NC 27707. Phone: (919) 294-7542. Fax: (919) 294-7661. E-mail: jenny.svarovskaia{at}gilead.com.

{triangledown} Published ahead of print on 27 September 2006.

Journal of Clinical Microbiology, November 2006, p. 4237-4241, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.01512-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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