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Journal of Clinical Microbiology, November 2006, p. 4246-4249, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00091-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Cryptosporidium spp. from Children in Kolkata, India{triangledown}

Pradeep Das,1* Seuli Saha Roy,2 Kakali MitraDhar,2 Phalguni Dutta,2 Mihir K. Bhattacharya,2 Abhik Sen,2 Sandipan Ganguly,2 Sujit K. Bhattacharya,2 Altaf A. Lal,3 and Lihua Xiao3

Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800 007, India,1 National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700 010, India,2 National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Highway, Atlanta, Georgia 303343

Received 16 January 2006/ Returned for modification 24 March 2006/ Accepted 30 August 2006

The intracellular parasite Cryptosporidium is responsible for severe diarrhea in immunocompromised persons in developing countries. Few studies on the characterization of the parasite in India are available. In this study, molecular characterization of the parasite from diarrheic children was carried out by PCR-restriction fragment length polymorphism analysis. At least three genotypes were identified. Out of 40 positive samples, 35 were positive for C. hominis, 4 were positive for C. parvum, and 1 was positive for C. felis. This study clearly suggests that cryptosporidiosis in this region is caused largely by anthroponotic transmission.

* Corresponding author. Mailing address: Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800 007, India. Phone: 91-612-2631565. Fax: 91-612-2634379. E-mail: dasp{at}cal2.vsnl.net.in.

{triangledown} Published ahead of print on 13 September 2006.

Journal of Clinical Microbiology, November 2006, p. 4246-4249, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00091-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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