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Journal of Clinical Microbiology, November 2006, p. 4255-4257, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00477-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Usefulness of Adult Bovine Serum for Helicobacter pylori Culture Media{triangledown}

Keigo Shibayama,1* Mitsuaki Nagasawa,2 Takafumi Ando,3 Masaaki Minami,3 Jun-ichi Wachino,1 Satowa Suzuki,1 and Yoshichika Arakawa1

Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan,1 Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Japan,2 Department of Gastroenterology, Nagoya University Graduate School of Medicine, Nagoya, Japan3

Received 6 March 2006/ Returned for modification 14 May 2006/ Accepted 27 August 2006

Fetal bovine serum (FBS) and adult bovine serum (BS) exhibited bactericidal activity against Helicobacter pylori at various levels, which were higher in BS than in FBS. The bactericidal activity was inactivated by heat treatment at 56°C for 30 min. Our results demonstrated that heat-treated BS is a useful serum source of H. pylori culture medium.

* Corresponding author. Mailing address: Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1, Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Phone: 81-42-561-0771. Fax: 81-42-561-7173. E-mail: keigo{at}nih.go.jp.

{triangledown} Published ahead of print on 6 September 2006.

Journal of Clinical Microbiology, November 2006, p. 4255-4257, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00477-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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