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Journal of Clinical Microbiology, November 2006, p. 4279-4282, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00496-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Limitations of DNA Sequencing for Diagnosis of a Mixed Infection by Two Fungi, Phaeoacremonium venezuelense and a Plectophomella sp., in a Transplant Recipient{triangledown}

Josep Guarro,1* Agenor Messias Silvestre Jr.,2 Gerard Verkley,3 Josep Cano,1 Olga Fischman Gompertz,2 Josepa Gené,1 Marília Marufuji Ogawa,4 Jane Tomimori-Yamashita,4 Solange Pistori Teixeira,4 and Fernando Augusto de Almeida3

Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain,1 Centraalbureau voor Schimmelculteres, Utrecht, The Netherlands,3 Departamento de Microbiologia, Imunologia e Parasitologia,2 Departamento de Dermatolologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil4

Received 7 March 2006/ Returned for modification 16 May 2006/ Accepted 7 September 2006

We describe a rare case of a subcutaneous infection by both Phaeoacremonium venezuelense and Plectophomella sp. in a Brazilian male. Sequencing of a ß-tubulin gene fragment allowed us to confirm the identification of the former. However, a similar procedure of sequencing rRNA gene fragments was not useful for the identification of the latter fungus.

* Corresponding author. Mailing address: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, 43201 Reus, Spain. Phone: 34 977 759 359. Fax: 34 977 759 322. E-mail: josep.guarro{at}urv.net.

{triangledown} Published ahead of print on 27 September 2006.

Journal of Clinical Microbiology, November 2006, p. 4279-4282, Vol. 44, No. 11
0095-1137/06/$08.00+0     doi:10.1128/JCM.00496-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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