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Journal of Clinical Microbiology, December 2006, p. 4336-4341, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.00759-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples{triangledown}

H. D. Roque,1 R. Vieira,2 S. Rato,1 and M. Luz-Martins1*

Laboratório de Micologia, Instituto de Higiene e Medicina Tropical/CREM, Universidade Nova de Lisboa, Lisboa, Portugal,1 Serviço de Dermatologia, Hospital Curry-Cabral, Lisboa, Portugal2

Received 11 April 2006/ Returned for modification 3 July 2006/ Accepted 12 September 2006

This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii, based on a global test of 182 strains/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.

* Corresponding author. Mailing address: Lab. Micologia, Instituto de Higiene e Medicina Tropical, R. da Junqueira, 1149-008 Lisboa, Portugal. Phone: 351213652600. Fax: 351213632105. E-mail: luz{at}ihmt.unl.pt.

{triangledown} Published ahead of print on 27 September 2006.

Journal of Clinical Microbiology, December 2006, p. 4336-4341, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.00759-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Shehata, A. S., Mukherjee, P. K., Aboulatta, H. N., El Akhras, A. I., Abbadi, S. H., Ghannoum, M. A. (2008). Single-Step PCR Using (GACA)4 Primer: Utility for Rapid Identification of Dermatophyte Species and Strains. J. Clin. Microbiol. 46: 2641-2645 [Abstract] [Full Text]  


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