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Candida pseudorugosa sp. nov., a Novel Yeast Species from Sputum

Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China,¹ Peking Union Medical College Hospital, Beijing 100730, China,² Graduate School of the Chinese Academy of Sciences, Beijing 100039, China³

Received 6 July 2006/ Returned for modification 6 August 2006/ Accepted 25 September 2006

Two yeast strains, strains XH 1026 and XH 1164, isolated from the sputum of an intensive care unit patient with acute pneumonia, were originally identified as Candida albicans and C. tropicalis, respectively. Sequence analysis of the 26S rRNA gene D1/D2 domain and the internal transcribed spacer (ITS) region indicated that the two strains represent a novel yeast species closely related to C. rogusa. The name Candida pseudorugosa sp. nov. is therefore proposed (type strain, AS 2.3107 [CBS 10433]). The new species is able to grow at 42°C and is resistant or insusceptible to amphotericin B (MIC, 2 µg/ml), caspofungin (MIC, 64 µg/ml), itraconazole (MIC, 1 µg/ml), and nystatin (MIC, 16 µg/ml); dose-dependent susceptible to fluconazole (MIC, 16 µg/ml); and susceptible to flucytosine (MIC, 0.125 µg/ml) and voriconazole (MIC, 0.125 to 0.25 µg/ml). The code for C. pseudorugosa sp. nov. provided by the API 20C AUX system is identical to that for C. rugosa. The colonies of the new species on CHROMagar Candida appear blue-green, similar to those of C. albicans. In addition to the molecular method based on D1/D2 domain or ITS region sequencing, use of the combination of the API system and CHROMagar Candida is helpful for the correct identification of C. pseudorugosa sp. nov.

Published ahead of print on 4 October 2006.

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