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Journal of Clinical Microbiology, December 2006, p. 4511-4514, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.00421-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genotyping of Clinical Herpes Simplex Virus Type 1 Isolates by Use of Restriction Enzymes{triangledown}

Peter Norberg,* Tomas Bergström, and Jan-Åke Liljeqvist

Department of Clinical Virology, Göteborg University, S-413 46 Göteborg, Sweden

Received 27 February 2006/ Returned for modification 5 June 2006/ Accepted 28 September 2006

Recently, three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates and laboratory strains. We utilized single-nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for the detection of recombinants.

* Corresponding author. Mailing address: Department of Virology, University of Göteborg, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden. Phone: (46) 31 3424615. Fax: (46) 31 3424960. E-mail: peter.norberg{at}microbio.gu.se.

{triangledown} Published ahead of print on 11 October 2006.

Journal of Clinical Microbiology, December 2006, p. 4511-4514, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.00421-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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