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Journal of Clinical Microbiology, December 2006, p. 4553-4558, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01887-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Screening of Topoisomerase Gene Mutations by a Novel Melting Curve Analysis Method for Early Warning of Fluoroquinolone-Resistant Streptococcus pneumoniae Emergence{triangledown}

Kazuko Y. Fukushima,1 Yoichi Hirakata,1,2* Kazuyuki Sugahara,1 Katsunori Yanagihara,1 Akira Kondo,1,2 Shigeru Kohno,2 and Shimeru Kamihira1

Department of Laboratory Medicine,1 Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan2

Received 11 September 2006/ Accepted 19 September 2006

We developed a real-time PCR assay combined with melting curve analysis for rapidly genotyping quinolone resistance-determining regions (QRDR) of topoisomerase genes in Streptococcus pneumoniae. This assay was not only accurate for the screening of fluoroquinolone (FQ) resistance but also relevant as an early warning system for detecting preexisting single QRDR mutations.

* Corresponding author. Mailing address: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan. Phone: 81-95-849-7276. Fax: 81-95-849-7285. E-mail: hirakata{at}nagasaki-u.ac.jp.

{triangledown} Published ahead of print on 4 October 2006.

Journal of Clinical Microbiology, December 2006, p. 4553-4558, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01887-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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