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Journal of Clinical Microbiology, December 2006, p. 4566-4568, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01916-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China as Determined by gyrA Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing{triangledown}

Ruiru Shi,1,2 Jianyuan Zhang,3 Chuanyou Li,3 Yuko Kazumi,1 and Isamu Sugawara1*

Mycobacterial Reference Center, The Research Institute of Tuberculosis, Tokyo, Japan,1 Henan Provincial Research Institute of Tuberculosis, Zhengzhou, China,2 Beijing Tuberculosis and Lung Tumor Research Institute, Beijing, China3

Received 14 September 2006/ Accepted 29 September 2006

A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates.

* Corresponding author. Mailing address: Mycobacterial Reference Center, The Research Institute of Tuberculosis, 3-1-24 Matsuyama, Kiyose, Tokyo 204-0022, Japan. Phone: 81 42 493 5075. Fax: 81 42 492 4600. E-mail: sugawara{at}jata.or.jp.

{triangledown} Published ahead of print on 11 October 2006.

Journal of Clinical Microbiology, December 2006, p. 4566-4568, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01916-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Xu, P., Li, X., Zhao, M., Gui, X., DeRiemer, K., Gagneux, S., Mei, J., Gao, Q. (2009). Prevalence of Fluoroquinolone Resistance among Tuberculosis Patients in Shanghai, China. Antimicrob. Agents Chemother. 53: 3170-3172 [Abstract] [Full Text]  
  • Mokrousov, I., Otten, T., Manicheva, O., Potapova, Y., Vishnevsky, B., Narvskaya, O., Rastogi, N. (2008). Molecular Characterization of Ofloxacin-Resistant Mycobacterium tuberculosis Strains from Russia. Antimicrob. Agents Chemother. 52: 2937-2939 [Abstract] [Full Text]  
  • Shi, R., Zhang, J., Otomo, K., Zhang, G., Sugawara, I. (2007). Lack of Correlation between embB Mutation and Ethambutol MIC in Mycobacterium tuberculosis Clinical Isolates from China. Antimicrob. Agents Chemother. 51: 4515-4517 [Abstract] [Full Text]  


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