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Journal of Clinical Microbiology, December 2006, p. 4572-4576, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01227-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples{triangledown}

Isabel Jado,1,2 Raquel Escudero,1 Horacio Gil,1 María Isabel Jiménez-Alonso,1,{dagger} Rita Sousa,3 Ana L. García-Pérez,4 Manuela Rodríguez-Vargas,1 Bruno Lobo,1 and Pedro Anda1*

Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,1 Unidad de Alertas y Emergencias, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,2 Centro de Estudos de Vectores e Doenças Infecciosas (CEVDI), Instituto Nacional de Saude, Lisboa, Portugal,3 Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER), Derio, Vizcaya, Spain4

Received 15 June 2006/ Returned for modification 25 July 2006/ Accepted 22 September 2006

We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.

* Corresponding author. Mailing address: Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain. Phone: 34 91 822 3601. Fax: 34 91 509 7966. E-mail: panda{at}isciii.es.

{triangledown} Published ahead of print on 11 October 2006.

{dagger} Present address: Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

Journal of Clinical Microbiology, December 2006, p. 4572-4576, Vol. 44, No. 12
0095-1137/06/$08.00+0     doi:10.1128/JCM.01227-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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