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Journal of Clinical Microbiology, February 2006, p. 297-301, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.297-301.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Restricted Infectivity of a Human-Lineage H3N2 Influenza A Virus in Pigs Is Hemagglutinin and Neuraminidase Gene Dependent

Gabriele A. Landolt, Alexander I. Karasin, Melissa M. Schutten, and Christopher W. Olsen*

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, Madison, Wisconsin

Received 23 July 2005/ Returned for modification 10 September 2005/ Accepted 13 November 2005

Influenza A viruses cause pandemics at sporadic intervals. Pandemic viruses can potentially be introduced into the human population through in toto transfer of an avian influenza virus or through reassortment between avian and human strains. Pigs are believed to play a central role in the creation of pandemic viruses through reassortment because of their susceptibility to infection with both avian and human influenza viruses. However, we recently found that a human-lineage H3N2 influenza virus was highly restricted in its ability to infect pigs after intranasal inoculation. We hypothesized that this restricted infectivity phenotype was controlled by the hemagglutinin (HA) and neuraminidase (NA). To test this, we infected pigs with reverse genetics-created HA plus NA reassortant viruses. Specifically, introduction of the HA and NA genes of a contemporary H3N2 swine virus into the genetic background of the wholly human virus resulted in a significant increase in virus shedding and pathogenicity. These data indicate that the HA/NA can play important roles in controlling human influenza virus infectivity in pigs. The results further support the premise that a barrier exists to human influenza virus infection in pigs, which may limit the role of pigs in pandemic virus creation through reassortment of human and avian influenza viruses.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin—Madison, 2015 Linden Drive, Madison, WI 53706. Phone: (608) 265-8681. Fax: (608) 263-0438. E-mail: olsenc{at}svm.vetmed.wisc.edu.

Journal of Clinical Microbiology, February 2006, p. 297-301, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.297-301.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Manzoor, R., Sakoda, Y., Nomura, N., Tsuda, Y., Ozaki, H., Okamatsu, M., Kida, H. (2009). PB2 Protein of a Highly Pathogenic Avian Influenza Virus Strain A/chicken/Yamaguchi/7/2004 (H5N1) Determines Its Replication Potential in Pigs. J. Virol. 83: 1572-1578 [Abstract] [Full Text]  


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