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Journal of Clinical Microbiology, February 2006, p. 334-339, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.334-339.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of the New GenoType Mycobacterium Assay for Identification of Mycobacterial Species

Cristina Russo,1 Enrico Tortoli,2* and Donato Menichella1

Microbiology Laboratory, Bambino Gesù Hospital, Rome,1 Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, Florence, Italy2

Received 12 August 2005/ Returned for modification 29 September 2005/ Accepted 6 December 2005

The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more frequently detected mycobacterial species and GenoType AS for less common species. The sensitivity and specificity were 97.9% and 92.4% for the CM kit and 99.3% and 99.4% for the AS kit. Our results show that the system may represent a useful tool to substantially enlarge the number of mycobacteria that can be reliably identified in clinical laboratories.

* Corresponding author. Mailing address: Centro Regionale di Riferimento per la Diagnostica dei Micobatteri, Piastra dei Servizi, Ospedale di Careggi, viale Morgagni 85, 50134 Firenze, Italy. Phone: 39-055-7949199. Fax: 39-055-7949010. E-mail: e.tortoli{at}libero.it.

Journal of Clinical Microbiology, February 2006, p. 334-339, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.334-339.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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