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*Trichomoniasis

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Journal of Clinical Microbiology, February 2006, p. 366-373, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.366-373.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of an Adaptation of a Commercially Available PCR Assay Aimed at Diagnosis of Chlamydia and Gonorrhea To Detect Trichomonas vaginalis in Urogenital Specimens

Barbara Van Der Pol,1* Colleen S. Kraft,2 and James A. Williams1

Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana,1 Department of Internal Medicine, Emory University School of Medicine, Atlanta, Georgia2

Received 22 July 2005/ Returned for modification 15 September 2005/ Accepted 24 November 2005

Trichomonas vaginalis PCR using reagents from a commercially available assay for Chlamydia trachomatis and Neisseria gonorrhoeae was evaluated for detection of infection in women and men attending a sexually transmitted disease clinic. Evaluations included three primer sets, endocervical swabs, vaginal swabs and urine, and various storage conditions. The TVK3/TVK7 primer set was optimal in our hands with sensitivities ranging from 69.5 to 96.8%. In all comparisons, T. vaginalis PCR performed better than routine diagnostics using microscopy for women and culture for men (P > 0.05). The assay performed well for all sample types tested, and vaginal swabs were stable for up to 7 days at ambient temperature. Using samples prepared for, and reagents from, the C. trachomatis-N. gonorrhoeae PCR assay allowed incorporation of T. vaginalis PCR diagnosis into routine clinical testing.

* Corresponding author. Mailing address: 545 N. Barnhill #435, Indianapolis, IN 46202. Phone: (317) 274-1422. Fax: (317) 278-1114. E-mail: bvanderp{at}iupui.edu.

Journal of Clinical Microbiology, February 2006, p. 366-373, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.366-373.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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