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Results of the First World Health Organization International Collaborative Study of Detection of Human Papillomavirus DNA

Delft Diagnostic Laboratory, Delft, The Netherlands,¹ Initiative for Vaccine Research, Department of Immunization, Vaccines and Biologicals, World Health Organization, Geneva, Switzerland,² Viral Quality Control Laboratory, Central Laboratory for Blood Transfusion, Diagnostic Division, Alkmaar, The Netherlands,³ Reference Center for Papillomaviruses, Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Heidelberg, Germany,⁴ Department of Molecular Genetics and Microbiology, School of Medicine, University of New Mexico, Albuquerque, New Mexico⁵

Received 18 August 2005/ Returned for modification 23 October 2005/ Accepted 14 November 2005

Twenty-nine laboratories in 12 countries participated in a study to assess the performance of various human papillomavirus (HPV) detection assays through the use of a recombinant HPV DNA standard reagent panel. The panel was designed by a group of HPV experts, and samples were prepared and distributed by the World Health Organization International Laboratory for Standards and Biologicals in The Netherlands. Each panel consisted of 24 coded samples including a dilution series for HPV types 16 and 18, alone or in combination with five other high-risk (HR) HPV types including HPV types 31, 33, 35, 45, and 52, the low-risk HPV type 6, and a negative control. Qualitative assays were generally consistent across laboratories, and most invalid results reflected a lack of HPV test sensitivity. The combined data sets had a proficiency for HPV 16 of 62.5% (15/24) and for HPV 18 of 73.9% (17/23). HPV 31 was the least accurately detected by participating laboratories. Approximately half of participating laboratories failed to detect high concentrations of HPV 31 and, to a lesser extent, to detect HPV types 35, 52, and 6. The panel sample materials offer a source of renewable and reproducible material that could be used in the future development of international standard reagents for calibration of HPV DNA assays and kits.

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