This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Joseph, J.
Right arrow Articles by Sharma, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Joseph, J.
Right arrow Articles by Sharma, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2006, p. 583-585, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.583-585.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of Different Stains for Microscopic Evaluation of Corneal Scrapings for Diagnosis of Microsporidial Keratitis

Joveeta Joseph,1 Somasheila Murthy,2 Prashant Garg,2 and Savitri Sharma1*

Jhaveri Microbiology Centre,1 Cornea and Anterior Segment Services, Hyderabad Eye Research Foundation, L.V. Prasad Eye Institute, L.V. Prasad Marg, Banjara Hills, Hyderabad 500034, India2

Received 28 November 2005/ Accepted 2 December 2005

Retrospective evaluation of potassium hydroxide plus calcofluor white (KOH+CFW), Gram, Giemsa, and modified Ziehl-Neelsen (1% H2SO4, cold) stains for the detection of microsporidia in corneal scrapings from 30 patients showed KOH+CFW and acid-fast stains to be most efficient (29/30 [96.7%] and 28/30 [93.3%], respectively) in the diagnosis of microsporidial keratitis.

* Corresponding author. Mailing address: Jhaveri Microbiology Centre, L.V. Prasad Eye Institute, L.V. Prasad Marg, Banjara Hills, Hyderabad 500034, India. Phone: 91-040-30612502. Fax: 91-040-23545305. E-mail: savitri{at}lvpei.org.

Journal of Clinical Microbiology, February 2006, p. 583-585, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.583-585.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Das, S, Sharma, S, Sahu, S K, Nayak, S S, Kar, S (2008). New microbial spectrum of epidemic keratoconjunctivitis: clinical and laboratory aspects of an outbreak. Br. J. Ophthalmol. 92: 861-862 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»