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Journal of Clinical Microbiology, February 2006, p. 583-585, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.583-585.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of Different Stains for Microscopic Evaluation of Corneal Scrapings for Diagnosis of Microsporidial Keratitis

Joveeta Joseph,1 Somasheila Murthy,2 Prashant Garg,2 and Savitri Sharma1*

Jhaveri Microbiology Centre,1 Cornea and Anterior Segment Services, Hyderabad Eye Research Foundation, L.V. Prasad Eye Institute, L.V. Prasad Marg, Banjara Hills, Hyderabad 500034, India2

Received 28 November 2005/ Accepted 2 December 2005

Retrospective evaluation of potassium hydroxide plus calcofluor white (KOH+CFW), Gram, Giemsa, and modified Ziehl-Neelsen (1% H2SO4, cold) stains for the detection of microsporidia in corneal scrapings from 30 patients showed KOH+CFW and acid-fast stains to be most efficient (29/30 [96.7%] and 28/30 [93.3%], respectively) in the diagnosis of microsporidial keratitis.

* Corresponding author. Mailing address: Jhaveri Microbiology Centre, L.V. Prasad Eye Institute, L.V. Prasad Marg, Banjara Hills, Hyderabad 500034, India. Phone: 91-040-30612502. Fax: 91-040-23545305. E-mail: savitri{at}lvpei.org.

Journal of Clinical Microbiology, February 2006, p. 583-585, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.583-585.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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