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Journal of Clinical Microbiology, February 2006, p. 598-603, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.598-603.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Proximal Region of the Gene Encoding Cytadherence-Related Protein Permits Molecular Typing of Mycoplasma genitalium Clinical Strains by PCR-Restriction Fragment Length Polymorphism

Oxana Musatovova, Caleb Herrera, and Joel B. Baseman*

Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio, MC 7758, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900

Received 26 July 2005/ Returned for modification 11 October 2005/ Accepted 17 November 2005

Restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified proximal region of the gene encoding cytadherence accessory protein P110 (MG192) revealed DNA sequence divergences among 54 Mycoplasma genitalium clinical strains isolated from the genitourinary tracts of women attending a sexually transmitted disease-related health clinic, plus one from the respiratory tract and one from synovial fluid. Seven of 56 (12.5%) strains exhibited RFLPs following digestion of the proximal region with restriction endonuclease MboI or RsaI, or both. No sequence variability was detected in the distal portion of the gene.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, MC 7758, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900. Phone: (210) 567-3939. Fax: (210) 567-6491. E-mail: baseman{at}uthscsa.edu.

Journal of Clinical Microbiology, February 2006, p. 598-603, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.598-603.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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