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Journal of Clinical Microbiology, February 2006, p. 619-621, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.619-621.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study

M. Aires-de-Sousa,1 K. Boye,2 H. de Lencastre,1 A. Deplano,3 M. C. Enright,4 J. Etienne,5 A. Friedrich,6 D. Harmsen,7* A. Holmes,8 X. W. Huijsdens,9 A. M. Kearns,8 A. Mellmann,6 H. Meugnier,5 J. K. Rasheed,10 E. Spalburg,9 B. Strommenger,11 M. J. Struelens,3 F. C. Tenover,10 J. Thomas,4 U. Vogel,12 H. Westh,2 J. Xu,13 and W. Witte11

Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal,1 Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark,2 Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium,3 Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom,4 Laboratoire Central de Microbiologie, Hôpital Edouard Herriot, Lyon, France,5 Institut für Hygiene, University Hospital Münster, Münster, Germany,6 Department of Periodontology, University Hospital Münster, Münster, Germany,7 Center for Infections, Health Protection Agency, London, United Kingdom,8 Diagnostic Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands,9 Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia,10 Robert-Koch-Institut, Wernigerode, Germany,11 Institut für Hygiene und Microbiologie, University Hospital Würzburg, Würzburg, Germany,12 State Key Laboratory of Infectious Diseases Prevention and Control, National Institute for Communicable Disease Prevention and Control, Beijing, People's Republic of China,13

Received 29 June 2005/ Returned for modification 2 September 2005/ Accepted 28 November 2005

Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

* Corresponding author. Mailing address: Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, D-48149 Münster, Germany. Phone: 49-251-9802875. Fax: 49-251-9802868. E-mail: dharmsen{at}uni-muenster.de.

Journal of Clinical Microbiology, February 2006, p. 619-621, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.619-621.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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