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Journal of Clinical Microbiology, February 2006, p. 622-624, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.622-624.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of Molecular Beacon Probes for Real-Time PCR Detection of Plasmodium falciparum and Other Plasmodium Species in Peripheral Blood Specimens

Sameer Elsayed,1,2,4* Katherine Plewes,4 Deirdre Church,1,3,4 Barbara Chow,4 and Kunyan Zhang1,2,3,4

Departments of Pathology & Laboratory Medicine,1 Microbiology & Infectious Diseases,2 Medicine, University of Calgary,3 Division of Microbiology, Calgary Laboratory Services, Calgary, Alberta, Canada4

Received 15 November 2005/ Returned for modification 3 December 2005/ Accepted 5 December 2005

We describe the development and evaluation of a novel pair of real-time, fluorescence-based PCR assays using molecular beacon probes for rapid, sensitive, and specific detection and quantification of Plasmodium falciparum and other Plasmodium species organisms.

* Corresponding author. Mailing address: Calgary Laboratory Services, 9-3535 Research Rd. NW, Calgary, Alberta, Canada T2L 2K8. Phone: (403) 770-3309. Fax: (403) 770-3347. E-mail: sameer.elsayed{at}cls.ab.ca.

Journal of Clinical Microbiology, February 2006, p. 622-624, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.622-624.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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