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Journal of Clinical Microbiology, February 2006, p. 669-672, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.669-672.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

CASE REPORT

Direct Detection of Cardiobacterium hominis in Serum from a Patient with Infective Endocarditis by Broad-Range Bacterial PCR

N. Gatselis,1 E. Malli,2 G. Papadamou,1 E. Petinaki,2 and G. N. Dalekos1*

Department of Medicine,1 Department of Microbiology, Medical School, University of Thessaly, Larissa, Greece2

Received 5 October 2005/ Returned for modification 13 November 2005/ Accepted 4 December 2005

Bacterial DNA was detected directly in the serum of a patient with endocarditis by broad-range 16S rRNA PCR followed by sequencing and analysis of the results by the BLAST search. Using these methods, Cardiobacterium hominis was identified in 2 days from the date of serum collection. The microorganism was also isolated and identified using conventional methods (bacterial culture and biochemical tests) 17 days from the date of sample collection. This is the first report showing the direct detection of C. hominis in a patient's serum using molecular-based methods, emphasizing their potential usefulness as additional and rapid diagnostic tools for the detection and identification of fastidious bacteria.

* Corresponding author. Mailing address: Department of Medicine, Medical School, University of Thessaly, Papakiriazi 22 str, 41222 Larissa, Greece. Phone: 30-2410-565251. Fax: 30-2410-565250. E-mail: dalekos{at}med.uth.gr.

Journal of Clinical Microbiology, February 2006, p. 669-672, Vol. 44, No. 2
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.2.669-672.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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