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Journal of Clinical Microbiology, March 2006, p. 1074-1076, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1074-1076.2006

Molecular Diagnosis of Bordetella pertussis Infection by Evaluation of Formalin-Fixed Tissue Specimens

Kathleen M. Tatti,1* Kai-Hui Wu,2 Gary N. Sanden,2 Patricia Greer,1 John Sumner,1 Jeannette Guarner,1 Christopher D. Paddock,1 and Sherif R. Zaki1

Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Viral and Rickettsial Disease, Infectious Disease Pathology Activity, Atlanta, Georgia 30333,1 Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Bacterial and Mycotic Disease, Meningitis and Special Pathogens Branch, Epidemiologic Investigations Laboratory, Atlanta, Georgia 303332

Received 12 December 2005/ Accepted 13 December 2005

Formalin-fixed lung or trachea tissue specimens from four infants and one adolescent who died of respiratory illness were tested for Bordetella pertussis by conventional and real-time PCR assays. B. pertussis was confirmed in all cases. PCR can be an invaluable retrospective diagnostic tool for evaluating archival tissues from patients with suspected fatal pertussis.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd., NE, Mailstop G30, Atlanta, GA 30333. Phone: (404) 639-3133. Fax: (404) 639-3043. E-mail: ket2{at}cdc.gov.

Journal of Clinical Microbiology, March 2006, p. 1074-1076, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1074-1076.2006




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