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Journal of Clinical Microbiology, March 2006, p. 1087-1089, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1087-1089.2006

PCR as a Confirmatory Technique for Laboratory Diagnosis of Malaria

Stephanie P. Johnston,1* Norman J. Pieniazek,1 Maniphet V. Xayavong,1,2 Susan B. Slemenda,1 Patricia P. Wilkins,1 and Alexandre J. da Silva1

Division of Parasitic Diseases, Centers for Disease Control and Prevention, and Public Health Service, U.S. Department of Health and Human ServicesAtlanta, Georgia,1 Georgia and Atlanta Research and Education Foundation, Atlanta, Georgia2

Received 13 October 2005/ Returned for modification 24 November 2005/ Accepted 13 December 2005

We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal.

* Corresponding author. Mailing address: Division of Parasitic Diseases, M.S. F-36, Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, GA 30341-3724. Phone: (770) 488-7044. Fax: (770) 488-3115. E-mail: sjohnston{at}cdc.gov.

Journal of Clinical Microbiology, March 2006, p. 1087-1089, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1087-1089.2006




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  • Glikman, D., Nguyen-Dinh, P., Roberts, J. M., Montgomery, C. P., Daum, R. S., Marcinak, J. F. (2007). Clinical Malaria and Sickle Cell Disease Among Multiple Family Members in Chicago, Illinois. Pediatrics 120: e745-e748 [Abstract] [Full Text]  
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