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Journal of Clinical Microbiology, March 2006, p. 1098-1100, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1098-1100.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of Testing Methods for Detection of Decreased Linezolid Susceptibility Due to G2576T Mutation of the 23S rRNA Gene in Enterococcus faecium and Enterococcus faecalis

Chao Qi,1,2* Xiaotian Zheng,1,3 Arlene Obias,2 Marc H. Scheetz,4 Michael Malczynski,2 and John R. Warren1,2

Department of Pathology, Northwestern University Feinberg School of Medicine,1 Clinical Microbiology Laboratory, Northwestern Memorial Hospital,2 Clinical Microbiology Laboratory, Children's Memorial Hospital,3 Department of Pharmacy, Northwestern Memorial Hospital, Chicago, Illinois4

Received 11 October 2005/ Returned for modification 1 December 2005/ Accepted 8 December 2005

E-test, Vitek 2, MicroScan, agar dilution, and disk diffusion were compared for detection of decreased linezolid susceptibility due to 23S rRNA gene G2576T mutation among 32 clinical Enterococcus strains initially reported as intermediate or resistant by E-test alone or Vitek 2 confirmed by E-test. Agar and broth dilution methods were in concordance with PCR detection of the mutation, and disk diffusion was somewhat less sensitive but equally specific.

* Corresponding author. Mailing address: Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611. Phone: (312) 926-2181. Fax: (312) 926-4559. E-mail: cqi451{at}northwestern.edu.

Journal of Clinical Microbiology, March 2006, p. 1098-1100, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1098-1100.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Werner, G., Bartel, M., Wellinghausen, N., Essig, A., Klare, I., Witte, W., Poppert, S. (2007). Detection of Mutations Conferring Resistance to Linezolid in Enterococcus spp. by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 45: 3421-3423 [Abstract] [Full Text]  
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