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Journal of Clinical Microbiology, March 2006, p. 1105-1107, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1105-1107.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Respiratory Syncytial Virus Detection by Remel Xpect, Binax Now RSV, Direct Immunofluorescent Staining, and Tissue Culture

Anita P. Borek, Susan H. Clemens, Valerie K. Gaskins, Deborah Z. Aird, and Alexandra Valsamakis*

Johns Hopkins Medical Institutions, 600 North Wolfe Street, Meyer Building, Room B1-193, Baltimore, Maryland 21287-7093

Received 7 November 2005/ Returned for modification 21 December 2005/ Accepted 27 December 2005

The performance characteristics of Xpect RSV (XP) and Binax Now RSV (BN) were compared to those of direct fluorescent-antibody staining and/or tissue culture for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirate and wash samples from children (n = 110) and adults (n = 66). The sensitivity, specificity, positive predictive value, and negative predictive value of XP were 75%, 98%, 95%, and 90%, respectively; and those of BN were 74%, 100%, 100%, and 90%, respectively. The performances of the assays were similar within a given age group and specimen type (nasopharyngeal aspirate or wash specimen). XP and BN are useful for screening for RSV in respiratory specimens when large volumes are tested or low levels of staffing occur.

* Corresponding author. Mailing address: Johns Hopkins Medical Institutions, Meyer Building, Room B1-193, 600 N. Wolfe St., Baltimore, MD 21287-7093. Phone: (410) 955-5077. Fax: (410) 614-8087. E-mail: avalsam1{at}jhmi.edu.

Journal of Clinical Microbiology, March 2006, p. 1105-1107, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1105-1107.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2006 by the American Society for Microbiology. All rights reserved.