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Journal of Clinical Microbiology, March 2006, p. 1115-1119, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.1115-1119.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of Sno Strip Filter-Paper Wicks for Collection of Genital-Tract Samples Allows Reproducible Determination of Human Immunodeficiency Virus Type 1 (HIV-1) RNA Viral Load with a Commercial HIV-1 Viral Load Assay

Diagnostic Virology and Reference Laboratory, Providence Health Care, and University of British Columbia, Vancouver, Canada,¹ Department of Obstetrics & Gynecology, University of British Columbia, Vancouver, Canada,² Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology, University of British Columbia, Vancouver, Canada,³ Diagnostic Virology and Reference Laboratory, St. Paul's Hospital, University of British Columbia, Vancouver, Canada,⁴ Department of Medicine, Royal Columbian Hospital, Vancouver, Canada,⁵ British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada,⁶ Centre for Excellence in HIV/AIDS, Vancouver, Canada,⁷ Oak Tree Clinic, Children's and Women's Health Centre of British Columbia, Vancouver, Canada⁸

Received 22 October 2004/ Returned for modification 30 March 2005/ Accepted 30 October 2005

To assess the reproducibility of measurements of cervical and vaginal human immunodeficiency virus (HIV) viral load, 92 duplicate cervical and 88 duplicate vaginal samples were collected from 13 HIV-infected women using Sno Strip filter-paper wicks. RNA was eluted from the strips, extracted, and assayed using a modified protocol for the Roche Cobas Amplicor HIV-1 Monitor assay. Pearson's correlation coefficient (R), coefficient of determination (D), and Bland-Altman plots (BA) were used to compare paired log₁₀-transformed viral loads. Analysis of duplicate same-site samples showed good reproducibility (cervix: R = 0.72, D = 52%, BA = 89% within range; vagina: R = 0.72, D = 51%, BA = 87% within range); paired cervix/vagina measurements showed moderate correlation only (R = 0.56; D = 31.3%). Standardized sample collection and simple modification of the Roche Cobas Amplicor HIV-1 Monitor assay allows reproducible measurement of genital viral load.