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Differentiation of Species Combined into the Burkholderia cepacia Complex and Related Taxa on the Basis of Their Fatty Acid Patterns

Eva Krejí^1,2* and Reiner M. Kroppenstedt²

Institute of Public Health, Centre for Microbiology, Parasitology and Immunology, Partyzánské nám. 7, 702 00 Ostrava, Czech Republic,¹ DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, 38124 Braunschweig, Germany²

Received 7 July 2005/ Returned for modification 29 August 2005/ Accepted 5 January 2006

Using the established commercial system Sherlock (MIDI, Inc.), cellular fatty acid methyl ester analysis for differentiation among Burkholderia cepacia complex species was proven. The identification key based on the diagnostic fatty acids is able to discern phenotypically related Ralstonia pickettii and Pandoraea spp. and further distinguish Burkholderia pyrrocinia, Burkholderia ambifaria, and Burkholderia vietnamiensis.

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