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Journal of Clinical Microbiology, March 2006, p. 688-692, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.688-692.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multicenter Laboratory Validation of the BACTEC MGIT 960 Technique for Testing Susceptibilities of Mycobacterium tuberculosis to Classical Second-Line Drugs and Newer Antimicrobials

Sabine Rüsch-Gerdes,1* Gaby E. Pfyffer,2 Manuel Casal,3 Maureen Chadwick,4 and Salman Siddiqi5

National Reference Center for Mycobacteria, Forschungszentrum Borstel, Borstel, Germany,1 Department of Medical Microbiology, Luzern General Hospital, Lucerne, Switzerland,2 Mycobacteria Reference Center, Faculty of Medicine, University of Cordoba, Cordoba, Spain,3 Royal Brompton Hospital, London, United Kingdom,4 Becton Dickinson Diagnostic Systems, Sparks, Maryland5

Received 5 September 2005/ Returned for modification 28 October 2005/ Accepted 13 December 2005

The BACTEC MGIT 960 system, a fully automated, nonradiometric, noninvasive system for detection and drug susceptibility testing of mycobacteria, was evaluated for the ability to test susceptibilities to second-line drugs. In this study, which was carried out in three phases (phase I, mostly susceptible strains; phase II, mostly resistant strains; phase III, final testing of the optimal drug concentrations found in phases I and II), we established the critical concentrations for seven drugs to be tested in the BACTEC MGIT 960 system compared to the BACTEC 460TB system. The critical concentrations for the seven drugs used in the MGIT 960 system are as follows: amikacin, 1.0 µg/ml; capreomycin, 2.5 µg/ml; ethionamide, 5.0 µg/ml; protionamide, 2.5 µg/ml; ofloxacin, 2.0 µg/ml; rifabutin, 0.5 µg/ml; linezolid, 1.0 µg/ml. Our results demonstrate that the BACTEC MGIT 960 system is an accurate method for rapid testing of the susceptibilities of Mycobacterium tuberculosis to second-line drugs.

* Corresponding author. Mailing address: Forschungszentrum Borstel, National Reference Center for Mycobacteria, Parkallee 18, D-23845 Borstel, Germany. Phone: (49) 4537-188213. Fax: (49) 4537-188311. E-mail: srueschg{at}fz-borstel.de.

Journal of Clinical Microbiology, March 2006, p. 688-692, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.688-692.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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