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Journal of Clinical Microbiology, March 2006, p. 772-776, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.772-776.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Effects of Moraxella (Branhamella) ovis Culture Filtrates on Bovine Erythrocytes, Peripheral Mononuclear Cells, and Corneal Epithelial Cells{dagger}

Henry E. Cerny,1 Douglas G. Rogers,2 Jeffrey T. Gray,3 David R. Smith,2 and Susanne Hinkley2*

Crete Veterinary Clinic, Crete, Nebraska 68333-0008,1 Department of Veterinary and Biomedical Sciences, University of Nebraska—Lincoln, Lincoln, Nebraska 68583-0907,2 Department of Pathobiology, University of Guelph, Ontario Veterinary College, Guelph, Ontario, Canada N1G 2W13

Received 29 September 2003/ Returned for modification 14 April 2004/ Accepted 14 December 2005

Infectious bovine keratoconjunctivitis (IBK) is a highly contagious ocular disease that affects cattle of all ages and that occurs worldwide. Piliated hemolytic Moraxella bovis is recognized as the etiologic agent of IBK. According to data from the Nebraska Veterinary Diagnostic Laboratory System, however, Moraxella (Branhamella) ovis has been isolated with increasing frequency from cattle affected with IBK. The objective of this study was, therefore, to examine M. ovis field isolates for the presence of the putative virulence factors of M. bovis. Culture filtrates from selected M. ovis field isolates demonstrated hemolytic activity on bovine erythrocytes and cytotoxic activity on bovine peripheral blood mononuclear cells and corneal epithelial cells. The hemolytic activity of the culture filtrates was attenuated after heat treatment. Polyclonal antibodies raised against the M. bovis hemolysin-cytotoxin also recognized a protein of approximately 98 kDa in a Western blot assay. These data indicate that the M. ovis field isolates examined produce one or more heat-labile exotoxins and may suggest that M. ovis plays a role in the pathogenesis of IBK.

* Corresponding author. Present address: GeneSeek, Inc., 4711 Innovation Drive, Lincoln, NE 68521. Phone: (402) 435-0665. Fax: (402) 435-0664. E-mail: shinkley{at}geneseek.com.

{dagger} This article is published as ARD Journal Series number 13909, with the approval of the University of Nebraska Agricultural Research Division.

Journal of Clinical Microbiology, March 2006, p. 772-776, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.772-776.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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