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Journal of Clinical Microbiology, March 2006, p. 783-789, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.783-789.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Isolation of the Genome Sequence Strain Mycobacterium avium 104 from Multiple Patients over a 17-Year Period

Kathleen L. Horan,1* Robert Freeman,1 Kris Weigel,1 Makeda Semret,2 Stacy Pfaller,3 Terry C. Covert,3 Dick van Soolingen,4 Sylvia C. Leão,5 Marcel A. Behr,2 and Gerard A. Cangelosi1

Seattle Biomedical Research Institute, Seattle, Washington,1 McGill University Health Center, Montreal, Canada,2 U.S. Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, Ohio,3 National Institute of Public Health and the Environment, Bilthoven, The Netherlands,4 Departmento de Microbiologia, Immunologia e Parasitologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, UNIFESP-EPM, São Paulo, Brazil5

Received 2 November 2005/ Returned for modification 14 December 2005/ Accepted 28 December 2005

The genome sequence strain 104 of the opportunistic pathogen Mycobacterium avium was isolated from an adult AIDS patient in Southern California in 1983. Isolates of non-paratuberculosis M. avium from 207 other patients in Southern California and elsewhere were examined for genotypic identity to strain 104. This process was facilitated by the use of a novel two-step approach. In the first step, all 208 strains in the sample were subjected to a high-throughput, large sequence polymorphism (LSP)-based genotyping test, in which DNA from each strain was tested by PCR for the presence or absence of 4 hypervariable genomic regions. Nineteen isolates exhibited an LSP type that resembled that of strain 104. This subset of 19 isolates was then subjected to high-resolution repetitive sequence-based PCR typing, which identified 10 isolates within the subset that were genotypically identical to strain 104. These isolates came from 10 different patients at 5 clinical sites in the western United States, and they were isolated over a 17-year time span. Therefore, the sequenced genome of M. avium strain 104 has been associated with disease in multiple patients in the western United States. Although M. avium is known for its genetic plasticity, these observations also show that strains of the pathogen can be genotypically stable over extended time periods.

* Corresponding author. Mailing address: Seattle Biomedical Research Institute, 307 Westlake Avenue N, Suite 500, Seattle, WA 98109. Phone: (206) 256-7200. Fax: (206) 256-7229. E-mail: kthoran{at}u.washington.edu.

Journal of Clinical Microbiology, March 2006, p. 783-789, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.783-789.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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