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Journal of Clinical Microbiology, March 2006, p. 888-891, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.888-891.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Reactivity of Antibodies from Syphilis Patients to a Protein Array Representing the Treponema pallidum Proteome

Mary Beth Brinkman,^1, Matthew McKevitt,^1, Melanie McLoughlin,² Carla Perez,¹ Jerrilyn Howell,² George M. Weinstock,⁴ Steven J. Norris,^2,3 and Timothy Palzkill^1*

Department of Molecular Virology and Microbiology,¹ Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza,⁴ Department of Pathology and Laboratory Medicine,² Department of Microbiology and Molecular Genetics, University of Texas—Houston Medical School, 6431 Fannin Street, Houston, Texas 77030³

Received 1 November 2005/ Returned for modification 22 December 2005/ Accepted 10 January 2006

To identify antigens important in the human immune response to syphilis, the serum antibody reactivity of syphilitic patients was examined with 908 of the 1,039 proteins in the proteome of Treponema pallidum subsp. pallidum using a protein array enzyme-linked immunosorbent assay. Thirty-four proteins exhibited significant reactivity when assayed with human sera from patients in the early latent stage of syphilis. A subset of antigens identified were further scrutinized for antibody reactivity at primary, secondary, and latent disease stages, and the results demonstrate that the humoral immune response to individual T. pallidum proteins develops at different rates during the time course of infection.

Supplemental material for this article may be found at http://jcm.asm.org/.

These authors contributed equally to the work.

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