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Journal of Clinical Microbiology, March 2006, p. 950-956, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.950-956.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Antigenic Properties and Diagnostic Potential of African Swine Fever Virus Protein pp62 Expressed in Insect Cells

Carmina Gallardo,1 Esther Blanco,1* Javier M. Rodríguez,2 Angel L. Carrascosa,2 and Jose Manuel Sanchez-Vizcaino1,{dagger}

CISA-INIA, Valdeolmos, Madrid 28130, Spain,1 CBMSO, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain2

Received 20 July 2005/ Returned for modification 28 October 2005/ Accepted 23 December 2005

African swine fever (ASF) is an infectious and economically important disease of domestic pigs. The absence of vaccine renders the diagnostic test the only tool that can be used for the control of new outbreaks of the disease. At present, the enzyme-linked immunosorbent assay (ELISA) test is the most useful method for large-scale ASF serological studies, although false positives have been detected, mainly on poorly preserved sera. In order to improve the current diagnostic test available for ASF, we have studied the antigenic properties of the ASF virus polyprotein pp62 and its suitability for use in a novel ELISA. Two well-known antigenic proteins of ASF virus, p32 and p54, were also included in this study. These proteins were expressed in the baculovirus expression system and used as antigens in ASF serological tests. Our results indicate that the use of these recombinant proteins as antigens in the ELISAs improves the sensitivity and specificity obtained with the conventional diagnosis test used to detect antibodies against ASF virus. Furthermore, the use of polyprotein pp62 in an ELISA for testing poorly preserved sera allows performance of the diagnosis of ASF without the need to confirm the results by the immunoblot test. These features make pp62 one of the most interesting viral proteins to be used for serological ASF diagnosis.

* Corresponding author. Mailing address: CISA-INIA, Carretera Algete a El Casar, sn. Valdeolmos, Madrid 28130, Spain. Phone: 34-91-6202300. Fax: 34-91-6202247. E-mail: blanco{at}inia.es.

{dagger} Present address: Departamento Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.

Journal of Clinical Microbiology, March 2006, p. 950-956, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.950-956.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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Copyright © 2006 by the American Society for Microbiology. All rights reserved.