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Journal of Clinical Microbiology, March 2006, p. 981-991, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.981-991.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Analysis of the Antibody Response to an Immunodominant Epitope of the Envelope Glycoprotein of a Lentivirus and Its Diagnostic Potential

Franca Mordasini, Hans-Rudolf Vogt, Marie-Luise Zahno, Ariane Maeschli, Chiara Nenci, Reto Zanoni, Ernst Peterhans, and Giuseppe Bertoni*

Institute of Veterinary Virology, CH-3012 Bern, Switzerland

Received 11 October 2005/ Returned for modification 2 December 2005/ Accepted 9 January 2006

The envelope glycoprotein of small ruminant lentiviruses (SRLV) is a major target of the humoral immune response and contains several linear B-cell epitopes. We amplified and sequenced the genomic segment encoding the SU5 antigenic site of the envelope glycoprotein of several SRLV field isolates. With synthetic peptides based on the deduced amino acid sequences of SU5 in an enzyme-linked immunosorbent assay (ELISA), we have (i) proved the immunodominance of this region regardless of its high variability, (ii) defined the epitopes encompassed by SU5, (iii) illustrated the rapid and peculiar kinetics of seroconversion to this antigenic site, and (iv) shown the rapid and strong maturation of the avidity of the anti-SU5 antibody. Finally, we demonstrated the modular diagnostic potential of SU5 peptides. Under Swiss field conditions, the SU5 ELISA was shown to detect the majority of infected animals and, when applied in a molecular epidemiological context, to permit rapid phylogenetic classification of the infecting virus.

* Corresponding author. Mailing address: Institute of Veterinary Virology, Laenggass-Str. 122, CH-3012 Bern, Switzerland. Phone: 41 31 631 24 83. Fax: 41 31 631 25 34. E-mail: bertoni{at}ivv.unibe.ch.

Journal of Clinical Microbiology, March 2006, p. 981-991, Vol. 44, No. 3
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.3.981-991.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2006 by the American Society for Microbiology. All rights reserved.