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Journal of Clinical Microbiology, April 2006, p. 1209-1218, Vol. 44, No. 4
0095-1137/06/$08.00+0 doi:10.1128/JCM.44.4.1209-1218.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Use of Semiconductor-Based Oligonucleotide Microarrays for Influenza A Virus Subtype Identification and Sequencing
Michael J. Lodes,1,
*
Dominic Suciu,1,
Mark Elliott,1
Axel G. Stover,1
Marty Ross,1
Marcelo Caraballo,1
Kim Dix,1
James Crye,1
Richard J. Webby,2
Wanda J. Lyon,3
David L. Danley,1 and
Andrew McShea1
CombiMatrix Corporation, Mukilteo, Washington 98275,1
St. Jude Children's Research Hospital, Memphis, Tennessee 38105,2
AFIOH/SDE, Brooks City AFB, San Antonio, Texas 782353
Received 12 October 2005/
Returned for modification 24 November 2005/
Accepted 14 January 2006
In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. Also described is an array-based enzymatic assay that can be used to sequence portions of both genes or any other sequence of interest.
* Corresponding author. Mailing address: CombiMatrix Corporation, 6500 Harbour Heights Parkway, Suite 301, Mukilteo, WA 98275. Phone: (425) 493-2272. Fax: (425) 493-2010. E-mail:
mlodes{at}combimatrix.com.
M.J.L. and D.S. contributed equally to this study.
Journal of Clinical Microbiology, April 2006, p. 1209-1218, Vol. 44, No. 4
0095-1137/06/$08.00+0 doi:10.1128/JCM.44.4.1209-1218.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.
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