This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adékambi, T.
Right arrow Articles by Drancourt, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adékambi, T.
Right arrow Articles by Drancourt, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2006, p. 1268-1273, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1268-1273.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Description of Mycobacterium conceptionense sp. nov., a Mycobacterium fortuitum Group Organism Isolated from a Posttraumatic Osteitis Inflammation

Toïdi Adékambi,1 Andréas Stein,1,2 Joseph Carvajal,1 Didier Raoult,1 and Michel Drancourt1*

Unité des Rickettsies, CNRS UMR 6020 IFR 48, Faculté de Médecine, Université de la Méditerranée, Marseille, France,1 Service de Médecine Interne et Maladies Infectieuses, Assistance Publique-Hôpitaux de Marseille Conception, Marseille, France2

Received 18 September 2005/ Returned for modification 23 November 2005/ Accepted 25 January 2006

A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37°C. 16S rRNA sequence analyses over 1,483 bp showed that they were identical and shared 99.7% (4-bp difference) sequence similarity with that of Mycobacterium porcinum, the most closely related species. Partial rpoB (723 bp) sequence analyses showed that the isolates shared 97.0% sequence similarity with that of M. porcinum. Further polyphasic approaches, including biochemical tests, antimicrobial susceptibility analyses, and hsp65, sodA, and recA gene sequence analysis, as well as % G+C determination and cell wall fatty acid composition analysis supported the evidence that these isolates were representative of a new species. Phylogenetic analyses showed the close relationship with M. porcinum in the Mycobacterium fortuitum group. The isolates were susceptible to most antibiotics and exhibited evidence for penicillinase activity, in contrast to M. porcinum. We propose the name Mycobacterium conceptionense sp. nov. for this new species associated with posttraumatic osteitis. The type strain is D16T (equivalent to CIP 108544T and CCUG 50187T).

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27, Boulevard Jean Moulin, 13385 Marseille Cedex 05, France. Phone: 33 04 91 32 43 75. Fax: 33 04 91 38 77 72. E-mail: Michel.Drancourt{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, April 2006, p. 1268-1273, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1268-1273.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Richardson, E. T., Samson, D., Banaei, N. (2009). Rapid Identification of Mycobacterium tuberculosis and Nontuberculous Mycobacteria by Multiplex, Real-Time PCR. J. Clin. Microbiol. 47: 1497-1502 [Abstract] [Full Text]  
  • Adekambi, T., Shinnick, T. M., Raoult, D., Drancourt, M. (2008). Complete rpoB gene sequencing as a suitable supplement to DNA-DNA hybridization for bacterial species and genus delineation. Int. J. Syst. Evol. Microbiol. 58: 1807-1814 [Abstract] [Full Text]  
  • Lamy, B., Marchandin, H., Hamitouche, K., Laurent, F. (2008). Mycobacterium setense sp. nov., a Mycobacterium fortuitum-group organism isolated from a patient with soft tissue infection and osteitis. Int. J. Syst. Evol. Microbiol. 58: 486-490 [Abstract] [Full Text]  
  • Serra, C., Loi, G., Saddi, B., Pautasso, M., Manzin, A. (2007). Unusual Clinical Presentation of Mycobacterium fortuitum Infection in an Immunocompetent Woman. J. Clin. Microbiol. 45: 1663-1665 [Abstract] [Full Text]  
  • Adekambi, T., Raoult, D., Drancourt, M. (2006). Mycobacterium barrassiae sp. nov., a Mycobacterium moriokaense Group Species Associated with Chronic Pneumonia.. J. Clin. Microbiol. 44: 3493-3498 [Abstract] [Full Text]  
  • (2006). List of new names and new combinations previously effectively, but not validly, published.. Int. J. Syst. Evol. Microbiol. 56: 2025-2027 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»