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Presence of E6 and E7 mRNA from Human Papillomavirus Types 16, 18, 31, 33, and 45 in the Majority of Cervical Carcinomas

Irene Kraus,^1, Tor Molden,^1,* Ruth Holm,² A. Kathrine Lie,² Frank Karlsen,³ Gunnar B. Kristensen,⁴ and Hanne Skomedal³

Institute of Pathology, National University Hospital, 0027 Oslo, Norway,¹ Department of Pathology, Norwegian Radium Hospital, 0310 Oslo, Norway,² NorChip AS, 3490 Klokkarstua, Norway,³ Department of Gynaecologic Oncology, Norwegian Radium Hospital, 0310 Oslo, Norway⁴

Received 12 September 2005/ Returned for modification 12 December 2005/ Accepted 13 January 2006

The oncogenic potential of the human papillomavirus (HPV) early genes E6 and E7 is well established and a source of interest with regard to HPV testing for cervical carcinoma. Here we present a study performed with 204 histologically confirmed invasive cervical squamous cell carcinomas (SCCs) in which we evaluated the HPV E6 and E7 mRNA detection assay PreTect HPV-Proofer for detection of high-risk HPV types 16, 18, 31, 33, and 45. For further evaluation, detection of E6 and E7 mRNA from HPV types 35, 52, and 58 by real-time multiplex nucleic acid sequence-based amplification was also included. For comparison and to assess the overall prevalence of various HPV types, samples were also tested for HPV DNA by both consensus and type-specific PCR, reverse line blotting, sequencing, and in situ hybridization. The overall prevalence of HPV was 97%. HPV E6 and E7 transcripts were detected in 188 of 204 (92%) biopsy specimens, of which 181 contained one of the following HPV types: 16, 18, 31, 33, or 45. Consensus PCR and type-specific PCR detected HPV in 187 of 204 and 188 of 204 (92%) specimens, respectively. In conclusion, this study verifies the presence of HPV E6 and E7 mRNA in SCCs and demonstrates that HPV infections among Norwegian women with SCCs are limited mainly to the five high-risk types, 16, 18, 31, 33, and 45. This, together with the fact that PreTect HPV-Proofer detects the HPV oncogenic transcripts, suggests that the assay is a valuable approach in the field of HPV detection in cervical carcinoma.

Irene Kraus and Tor Molden contributed equally to this work.

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