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Journal of Clinical Microbiology, April 2006, p. 1352-1358, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1352-1358.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Occurrence of Overlooked Zoonotic Tuberculosis: Detection of Mycobacterium bovis in Human Cerebrospinal Fluid

N. P. Shah,^1, A. Singhal,^1, A. Jain,^1, P. Kumar,¹ S. S. Uppal,² M. V. P. Srivatsava,³ and H. K. Prasad^1*

Departments of Biotechnology,¹ Neurology, All India Institute of Medical Sciences,³ Department of Pediatrics, Safdarjung Hospital, New Delhi 110029, India²

Received 27 August 2005/ Returned for modification 28 October 2005/ Accepted 11 December 2005

The paucibacillary nature of the cerebrospinal fluid (CSF) has been a major obstacle in the diagnosis of human tuberculous meningitis (TBM). This study shows that with molecular techniques direct precise determination to the species level of mycobacterial pathogens can be made. The present report describes the utility of a nested PCR (N-PCR) assay (A. Mishra, A. Singhal, D. S. Chauhan, V. M. Katoch, K. Srivastava, S. S. Thakral, S. S. Bharadwaj, V. Sreenivas, and H. K. Prasad, J. Clin. Microbiol. 43:5670-5678, 2005) in detecting M. tuberculosis and M. bovis in human CSF. In 2.8% (6/212) of the samples, M. tuberculosis was detected, and in 17% (36/212), M. bovis was detected. Mixed infection was observed in 22 samples. Comparative analysis of clinical diagnosis, smear microscopy, and N-PCR in 69 patients (TBM, 25; non-TBM, 44) showed that the sensitivity of N-PCR (61.5%) was greater than that of smear microscopy (38.4%). Determination to the species level is important from the viewpoint of determining the prevalence of these mycobacteria in a community and would influence strategies currently adopted for the prevention of tuberculosis.

N.P.S. and A.S. contributed equally to this report.

Present address: Department of Biochemistry, Lady Hardinge Medical College, New Delhi 110001, India.

This article has been cited by other articles:

• Kumar, P., Srivatsava, M. V. P., Singh, S., Prasad, H. K. (2008). Filtration of Cerebrospinal Fluid Improves Isolation of Mycobacteria. J. Clin. Microbiol. 46: 2824-2825 [Full Text]  
• Ritacco, V., Kantor, I. N. d. (2007). Simultaneous Detection of Mycobacterium bovis and Mycobacterium tuberculosis in Human Cerebrospinal Fluid. J. Clin. Microbiol. 45: 684-684 [Full Text]